IV. CHARACTERIZATION OF CHOLINESTERASE ACTIVITY IN GREEN MUSSEL (Perna viridis) AS A POTENTIAL BIOMARKER IN MARINE BIOMONITORING
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چکیده
IV. CHARACTERIZATION OF CHOLINESTERASE ACTIVITY IN GREEN MUSSEL (Perna viridis) AS A POTENTIAL BIOMARKER IN MARINE BIOMONITORING 4.1. Abstract Green mussels (Perna viridis) play an important economic and ecological role in the coastal areas of Indonesia. P. viridis has been used as an eco-sentinel organism for marine biomonitoring programme in Asia regions. To magnify its competency as a relevant organism in biomonitoring, we characterized the cholinesterases (ChEs) of P. viridis from a selected coastal area of Indonesia. Dissected organs which were gill, foot, mantle and posterior adductor muscle (PAM) were examined for substrate specificity and inhibitors sensitivity using selective and non-selective substrates and inhibitors. The highest level of the ChE activity was observed in gill and followed by foot, mantle and PAM. The substrate specificity approach using various Alkylthiocholines indicated that the ChEs of P. viridis constitute an acetylcholinesterase (AChE) and a butyrylcholinesterase (BuChE). Likewise, the inhibitors sensitivity approach using eserine, BW284C51, and iso-OMPA sustained the previous approach which recorded typical AChE and atypical BuChE. The results suggested that the P. viridis gill is the most suitable organ for employing the ChE activity as a biomarker in marine monitoring.
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